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Figure 3. Cep152 and Plk4 interaction and localization. (A) Schematic of Cep152 full length (FL) and deletion constructs showing the ability to interact with Plk4 and Cep152 and to localize to the centrosome (Cent). Interactions were determined by coimmunoprecipitation of GFP-Cep152 constructs from HEK293T lysates with Plk4-myc or Cep152-myc. Centrosome localization was tested by expression of fragments as GFP fusions in U2OS cells. Numbers indicate Cep152 amino acids. CR1 and CR2 indicate conserved domains in Cep152 orthologues; SMC-A and SMC-B indicate coiled-coil regions. (B) Schematic of Plk4 deletion constructs and summary of interactions with Cep152. Domains: Cry-pb, crypto Polo-box; Pb, Polo-box; KinD, kinase domain. Interactions were determined by coimmunoprecipitation of Plk4-myc fragments from HEK293T lysates with GFP-Cep152. (C, top) U2OS cells expressing GFP-Cep152 and Plk4-myc labeled with antibodies to GFP and myc. (bottom) Untransfected U2OS cells labeled with antibodies to Cep152 and Plk4. Insets show enlarged centrosomes. Bar, 5 µm. Image published in: Hatch EM et al. (2010) © 2010 Hatch et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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