XB-IMG-125553
Xenbase Image ID: 125553
|
Fig. 6. Interaction of tryptophan and related derivatives with PAT2. A. l-[3H]Proline (10 μM) uptake (pH 5.5, Na+-free) in PAT2-expressing oocytes in the absence (control) and presence of proline (Pro) or tryptophan and related derivatives (all 10 mM), 5-hydroxy-l-tryptophan (OH-Trp), α-methyl-d,l-tryptophan (Me-Trp), l-tryptophan (l-Trp), 1-methyl-l-tryptophan (1-Me-l-Trp), indole-3-propionic acid (3-IPA), tryptamine (Trypt), 1-methyl-d-tryptophan (1-Me-d-Trp), d-tryptophan (d-Trp) and serotonin (5-HT). Results are expressed as PAT2-specific uptake. Data are mean ± SEM (n = 19–20). ***, p < 0.001; ns, p > 0.05, versus control. B. l-[3H]Proline (10 μM) uptake (pH 5.5, Na+-free) in the absence and presence of 5-hydroxy-l-tryptophan (OH-Trp) or α-methyl-d,l-tryptophan (Me-Trp) (both 0.1–30 mM). Results are expressed as a percentage of the uptake in the absence of inhibitor (control). Data are mean ± SEM (n = 19–20). C. Representative current traces in PAT2-expressing or water-injected oocytes (pH 5.5, Na+-free) during exposure to 0.2 mM proline (Pro, open bar) in the presence or absence of 20 mM OH-Trp (solid bar) and 20 mM Me-Trp (hatched bar). D. Mean PAT2-specific current evoked by proline (0.2 mM, open column), OH-Trp (filled column) or Me-Trp (hatched column) (both 20 mM), as shown in part C. Data are mean ± SEM (n = 5–6). ***, p < 0.001; ns, p > 0.05, versus water-injected oocytes. E. Mean PAT2-mediated current (in part C) when oocytes are exposed to proline (0.2 mM) in the absence (Pro, open column) or presence of either OH-Trp (Pro + OH-Trp, filled column) or Me-Trp (Pro + Me-Trp, hatched column) (both 20 mM). Results are expressed as a percentage of the control (the current observed in the presence of 0.2 mM proline alone). ***, p < 0.001 versus control. Data are mean ± SEM (n = 5–6). F. Concentration-dependent inhibition by Me-Trp (1–30 mM) of PAT2-mediated proline-induced current (0.2 mM). Data are mean ± SEM (n = 5). Image published in: Edwards N et al. (2011) © 2011 Elsevier B. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |