XB-IMG-125727
Xenbase Image ID: 125727
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Figure 1. (A) Schematic representation of the pentose phosphate pathway. (B) Western blot with anti-Xenopus G6PD antibodies on Xenopus egg extract, which was not depleted (input), mock depleted (mock) or G6PD depleted (ΔG6PD). (C) Kinetic readings of NADPH fluorescence in mock (triangles) or G6PD depleted (squares) extracts. Fluorescent intensity is indicated in arbitrary units (a.u.). (D) Xenopus egg extract was treated with 20 ng/μl of DSBs in the presence or absence of 5 mM caffeine or left untreated. The samples were then loaded on 10% SDS–PAGE and analysed by western blot with anti-p-Ser1981 ATM. (E) Kinetic readings of NADPH fluorescence in untreated extract (ctr) or DSB-treated extract (DSBs). (F) Xenopus egg extract was treated with or without 20 ng/μl DSBs for 10 min and then incubated with 2′-5′-ADP-sepharose beads or protein G-sepharose as a control. An aliquot of the eluted protein was loaded on a 10% SDS–PAGE and the gel stained with Coumassie blue. (G) A second aliquot was instead used to measure G6PD activity. (H) Xenopus egg extract was untreated (ctr), treated with 20 ng/μl DSBs alone (DSBs) or in combination with 10 μM ATMi (ATMi) or 5 mM caffeine (Caffeine). The samples were then subjected to 2′-5′-ADP-sepharose pull down. G6PD activity was measured on the proteins eluted from beads after the pull down. Graphs shown in this figure indicate average values of three or more experiments. Error bars indicate s.d. A full-colour version of this figure is available at The EMBO Journal Online. Image published in: Cosentino C et al. (2011) Copyright © 2011, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |