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Figure 4. Silencing of Hsp27 in human fibroblasts. (A) Cell transfected with control or Hsp27 targeting siRNA were lysed. Total protein extract was loaded on SDS–PAGE and analysed for Hsp27 content (lower panel) and tubulin (upper panel). (B) G6PD activity assay in cells where Hsp27 was silenced. Graph indicates average values from independent experiments. Error bars indicate s.d. Image published in: Cosentino C et al. (2011) Copyright © 2011, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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