Xenbase Image ID: 125855

Figure 1. Design strategy of β2-based Ion Channel-Coupled Receptors.ICCRs were formed by covalent linkage of GPCRs C-termini to Kir6.2 channel N-terminus. Helix H8 and β-bridge β1 are predicted from the β2AR (PDB code: 2RH1) and chimeric Kir3.1 (PDB code: 2QKS) structures, respectively. M2-K0–25 and D2-K0–25 are the ICCRs previously shown to be functional with 25 residues deleted from the Kir6.2 N-terminus. We used the same Kir6.2 deletion to build β2 ICCRs, with additional deletions in the receptor C-terminus. β2-K0–25 ICCR contains the full-length receptor, β2-K-62-25 and β2-K-72-25 are based on the β2AR deleted of 62 and 72 residues in its C-terminal domain to match the lengths of M2 and D2, respectively. Image published in: Caro LN et al. (2011) Caro et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page