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Figure 6. The stabilizing mutation E122 W weakens agonist-induced channel responses.(A) Location of Glu122 (in red) in the β2-adrenergic receptor structure. (B) Concentration-effect curves of isoproterenol on β2-K-62-25 and β2-K-72-25, unmodified (WT) and harboring mutation E122 W (all co-expressed with TMD0). Values are average of 5–14 measurements. Hill equation fits, represented as smooth lines, yielded EC50 of 149 nM, 247 nM, and 288 nM for β2-K-62-25, β2(E122 W)-K-62-25, and β2-K-72-25, respectively. h was 1.07, 1, and 1.18. Image published in: Caro LN et al. (2011) Caro et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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