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Figure 4. NeuroD1 antagonizes miR-124-induced cell proliferation. Transverse sections of the optic vesicle in stage 22/23 embryos injected with Pre-124 (0.025 pmol) and/or NeuroD1 (10 pg). Proliferzating cells were immunohistochemically stained with phosphohistone-H3 (pH3) antibody (red). Nuclei were labeled with Hoechst33 258 (blue). The pH3-positive cell ratio of transverse sections was shown in the bar graph (Mean ± SEM, 16 sections from four embryos). More proliferating cells in the brain and optic vesicle were detected in the Pre-124 group compared with the uninjected blank control. Injection of NeuroD1 mRNA alone had no obvious effect on cell proliferation. Co-injection with Pre-124 plus NeuroD1 restored cell proliferation to the level of the blank control. Scale bar: 100 μm. *P < 0.05; **P < 0.01. Image published in: Liu K et al. (2011) © The Author(s) 2010. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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