Xenbase Image ID: 126095

Fig. 1. Identification of XLAP2ω and XLAP2γ polypeptides immu-no-isolated from Xenopus egg extract. a Immunoblot from the immuno-isolation experiment with control and anti-XLAP2 Igs; staining with XLAP2-specific antibodies. Of the total material, 10% was loaded onto each lane (arrowheads positions of the 86-kDa XLAP2ω and 40-kDa XLAP2γ bands in control egg extract in the C lane and of isolated XLAP2ω polypeptide in the IP lane, C control egg extract, Ig-H heavy immunoglobulin chain, Ig-L light immunoglobulin chain, L loaded egg extract, W wash, FT flow through, IP immunoprecipitated proteins, M molecular-weight markers). b Fragments of silver-stained gel containing protein samples from the pull-down reaction (90% of the material was loaded). Gel pieces from boxes were excised and subjected to mass spectrometry analysis (LC/MS/MS). Molecular masses of reference proteins (in kDa) are shown. c Representations of XLAP2 protein isoforms translated in vitro from cDNA sequences found in Xenopus: XLAP2 clone 2 (AN: AF048815); XLAP2 clone 3 (AN: AF048816); XLAP2 clone 4 (AN: AF048817, Gant et al. 1999); XLAP2β (AN: Y17861, Lang et al. 1999); XLAP2ω (AN: AJ514937, Schoft et al. 2003). Identical regions are shaded similarly. Numbers above each representation indicate the amino-acid positions of domains. Sequences marked with letters are putative Xenopus-specific exons (Gant et al. 1999), which are absent from mammals and are positioned between exons 5 and 6 (A) or 8 and 9 of mouse cDNA (Berger et al. 1996). The lamin-binding domain is according to (Lang and Krohne 2003). Tryptic peptides identified by using LC/MS/MS are given below each representation. The 86-kDa polypeptide was identified as the translation product of two cDNAs: XLAP2 clone 2 (AF048815) and XLAP2ω (AJ514937). The 40-kDa polypeptide was determined as a member of XLAP2 family Image published in: Chmielewska M et al. (2011) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2011 Larger Image Printer Friendly View

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