Xenbase Image ID: 126098

Fig. 4. Intra-nuclear localization of XLAP2 as correlated to nuclear antigens and lipid membranes. XTC tissue-cultured cells were fixed with paraformaldehyde and probed for XLAP2 (green in b, red in e) or costained for XLAP2 (red in a, c, d) and lamin B2 (LAM B2, green in a, yellow in c, d) or costained for XLAP2 (green, f, g) and either p62-F/G nucleoporins (p62-Nup F/G, Ab 414, red, f) or phosphorylated histone H3 (red, g). Cellular membranes were visualized with dihydrocholecalciferol (DHCC) and DNA was counterstained with DAPI. a Various phenotypes of cultured cells presenting large nuclear channels (arrow) or nuclear dots (arrowheads) in which XLAP2 colocalizes with lamin B2. b Large channels (arrows) and smaller invaginations (arrowheads) are present on consecutive Z-sections of the cell nucleus from the bottom to the top. c, d XLAP2 staining correlates with lamin B2 and lipid membranes in NE. The nuclear territory contains nuclear dots/invaginations that colocalize with lamin B2 (arrowheads) or show a single XLAP2 signal (double arrowheads), forming channels (arrow) concomitant with the membrane signal (DHCC). e Cell with a large nuclear channel lacking DNA but possessing membrane signal (arrow). f, g Large nuclear invaginations or channels running from the bottom to the top of a nucleus. Images represent single Z-section (0.25 μm thick) from the nucleus, with the lines marking the surface of the Z-axis cross section through the nucleus. The cross sections from seven consecutive Z-stacks are shown bottom and right. Note that XLAP2 signal colocalizes with p62-F/G repeats containing nucleoporins (f) or with branches of the DNA-free tunnel in the nucleus (g). Bars 10 μm (a, b, e), 5 μm (c, d) Image published in: Chmielewska M et al. (2011) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2011 Larger Image Printer Friendly View

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