XB-IMG-126113
Xenbase Image ID: 126113
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FIG. 1. Whole-cell KATP currents for wild-type and mutant channels. A–C: Representative whole-cell current amplitudes evoked by repeated voltage steps from −10 to −30 mV for wild-type (A, WT), and homomeric (B, homE1506D) or heterozygous (C, hetE1506D) Kir6.2/SUR1-E1506D channels. The bars indicate application of 3 mmol/L azide, 0.34 mmol/L diazoxide (Dz), and 0.5 mmol/L tolbutamide (Tb). The dashed line (- - -) indicates the zero current level. Scale bars are 0.5 μA (y-axis) and 400 s (x-axis). D and E: Mean steady-state whole-cell KATP current amplitudes for wild-type homomeric (D) and heterozygous (E) mutant channels. Currents were evoked by a voltage step from –10 to –30 mV before (control, ■) and after (▨) application of 3 mmol/L azide, in the presence of 3 mmol/L azide plus 0.34 mmol/L Dz (□), and 3 azide + 0.5 mmol/L Tb (▧) for WT and SUR1 mutant channels, as indicated. The number of oocytes tested is given below the bars. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 against WT (Student t test). The error bars show the SEM. Image published in: Männikkö R et al. (2011) © 2011 by the American Diabetes Association. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |