XB-IMG-126114
Xenbase Image ID: 126114
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FIG. 2. ATP sensitivity of wild-type (WT) and mutant channels. A: Representative inside-out patch currents recorded in response to MgATP (100 μmol/L) for channels composed of WT Kir6.2 and WT or mutant SUR1, as indicated. The dashed lines (- - -) indicate the zero-current level. The solid lines (–––) indicate nucleotide application. B: Mean relationship between KATP current (I), expressed relative to that in the absence of nucleotide (Ic), and ATP concentration in the presence (B–D) or absence (E–G) of 2 mmol/L Mg2+ for WT (○, - - - ) and mutant (●, –––) channels. The lines are drawn to eq. 1, with the following parameters (in μmol/L for IC50): B–D: wild-type (n = 23), IC50 = 12.2, h = 1.05; E–G: WT (n = 7), IC50 = 8.3, h = 1.09. B: Kir6.2/SUR1-E1506D (n = 11), IC50 = 20.1, h = 1.05. C: Kir6.2/SUR1-E1506G (n = 10), IC50 = 18.8, h = 0.93. D: Kir6.2/SUR1-E1506K (n = 11), IC50 = 11.0, h = 1.14. E: Kir6.2/SUR1-E1506D (n = 7), IC50 = 8.2, h = 1.00. F: Kir6.2/SUR1-E1506G (n = 7), IC50 = 6.9, h = 0.86. G: SUR1-E1506K (n = 7), IC50 = 6.4, h = 1.00. Image published in: Männikkö R et al. (2011) © 2011 by the American Diabetes Association. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |