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FIG. 3. MgADP activation of wild-type (WT) and mutant channels. A: Representative inside-out patch currents recorded in response to application of MgADP (100 μmol/L) for channels composed of WT Kir6.2 and WT or mutant SUR1, as indicated. The dashed lines (- - -) indicate the zero-current level. The solid bars (■) indicate the application of nucleotide. Note that 100 μmol/L MgADP activates WT-SUR1 channels but blocks all the mutant E1506 channels. B and C: MgADP activation of WT or mutant Kir6.2/SUR1-E1506 channels, as indicated. The number of patches is indicated below the bars. B: KATP current (I) in the presence of 100 μmol/L MgADP is expressed relative to the current in the absence of nucleotides (IC). C: KATP current (I) in the presence of 100 μmol/L MgADP and 100 μmol/L MgATP is expressed relative to the current in the presence of 100 μmol/L MgATP alone (IATP). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 against wild type (Student t test). Statistical significance between mutant E1506 channels is reported in Supplementary Table 1. The error bars show the SEM. Image published in: Männikkö R et al. (2011) © 2011 by the American Diabetes Association. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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