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FIG. 5. Time course of MgADP and MgATP activation of KATP channels. Channels were composed of Kir6.2-G334D and wild-type (WT) or mutant SUR1 subunits, as indicated. Current activation and deactivation were fit with a single exponential function. The number of patches is indicated below the bars. Time constant of current activation (τon) by 100 μmol/L MgADP (A) or 100 μmol/L MgATP (B) is shown. Time constant of current deactivation (τoff) after removal of 100 μmol/L MgADP (C) or 100 μmol/L MgATP (D) is shown. *P ≤ 0.05 **P ≤ 0.01, ***P ≤ 0.001 against wild-type, (Student t test). Statistical significance between mutant E1506 channels is reported in Supplementary Table 1. The error bars show the SEM. E: Representative current traces for WT and Kir6.2/SUR1-E1506D (E1506D) channels. The horizontal bar indicates the duration of application of 100 μmol/L MgATP. The WT trace is interrupted to align the time point of ATP removal with that of the E1506D trace. Current amplitudes are normalized. Image published in: Männikkö R et al. (2011) © 2011 by the American Diabetes Association. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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