Xenbase Image ID: 126184

Figure 5. Tubular crystals of recombinant human AQP1.(A) Coomassie Blue-stained SDS/polyacrylamide gel of the purified HA-AQP1 used for 2D crystallization. Nonglycosylated HA-AQP1 migrates at ∼25 kDa while glycosylated proteins migrate above. (B) Negatively stained tubular crystals of HA-AQP1. The area marked by the white box was magnified and is displayed as inset: tubular crystals with a typical width of ∼0.11 µm are seen. (C) The power spectrum calculated from a flattened tubular HA-AQP1 crystal indicates regular order. Strong and weaker diffraction spots are marked by a circle and arrowheads, respectively. The scale bars represent 0.5 µm (B) and (50 Å)−1 (C). The frame size of the inset in (B) is 0.503×0.362 µm. Image published in: Bergeron MJ et al. (2011) Bergeron et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page