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Figure 3. Histone Modifications of transcriptionally repressed chromatin and constitutive heterochromatin in Xenopus embryos.Bar-Charts of repressive lysine methylation states on histone H3K9 and H4K20. A) Data from Orbi-Trap mass spectrometry - error bars: SD of two independent biological replicates. B) Data from MALDI-TOF mass spectrometry - error bars: SD of three independent biological replicates. C) Immunoblotting for total Histone H3 and histone H4 trimethylated at lysine 20. Left panel – western blot Odyssey infrared imaging signals; right panel – bar chart showing increase of H4K20me3 levels relative to total histone H3 protein during development. Abbreviations: unmod  =  unmodified peptide, Kac  =  acetylated lysine residue, Kme1  =  mono-methylated lysine residue, Kme2  =  di-methylated lysine residue, Kme3  =  tri-methylated lysine residue. Where applicable, p values are given by numbers above brackets to indicate significant differences in the abundance of a histone modification between samples. Image published in: Schneider TD et al. (2011) Schneider et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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