Xenbase Image ID: 126364

Figure 5. hPMC2 exonuclease activity is required for repair of estrogen-induced abasic sitesMCF10A cells were transfected with either hPMC2 miRNA that targets hPMC2 3'-UTR or hPMC2 miRNA along with plasmid expressing WT or the DM hPMC2. Cells were either untreated or treated with 50 nM E2 for 24 hours. Genomic DNA was isolated from cells and processed for Aldehyde-Reactive Probe (ARP) labeling as described in “Materials and Methods”. Columns represent the number of AP sites/105 base pair (bp) in control or E2-treated samples. Error bars indicate standard deviation of 2 independent experiments. a, significance (P < 0.05) vs. untreated cells; b, significance (P < 0.05) vs. control transfected cells with the same treatment. Image published in: Krishnamurthy N et al. (2011) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

Return to previous page