Xenbase Image ID: 126393

Figure 2. HKT1;1-mediated Na+ currents in E2586 wild-type (WT) and lack thereof in athkt1;1-4 GFP-labeled root stelar cell protoplasts.A) Typical whole-cell currents activated by rapid voltage ramps (5 mV ms−1, +99 to −141 mV) with the bath solution containing 0 (grey trace) or 50 mM NaCl (black trace) in wild-type E2586 root stelar cell protoplasts. B) Average current-voltage relationships recorded as in (A) from five wild-type E2586 protoplasts. Filled circles and open circles represent 0 mM and 50 mM NaCl in the bath respectively. The data are mean ± SE. The average current of −59 pA activated at a voltage of −140 mV corresponds to a current density of −35 mA m−2 with an average protoplast diameter of 23 µm. C) Typical whole-cell recordings using the same voltage ramps as in (A) with the bath solution containing 0 (grey trace) or 50 mM NaCl (black trace) in athkt1;1-4 loss-of-function mutant root stelar cell protoplasts. D) Average current-voltage relationships recorded as in (C) from five athkt1;1-4 mutant protoplasts. Filled circles and open circles represent 0 mM and 50 mM NaCl in the bath respectively. The data are mean ± SE. The bath solution contained 0/50 mM NaCl, 2 mM CaCl2, 5 mM MES, pH 5.7 adjusted with Tris. The pipette solution contained 50 mM NaCl, 1.3 mM CaCl2, 3 mM EGTA, 5 mM HEPES, pH 7.2 adjusted with Tris. The osmolalities of bath and pipette solutions were adjusted to 290–300 mmol·Kg−1 with D-mannitol. Liquid junction potential (LJP) was +0.3 mV for 50 mM [Na+]o and −12 mV for 0 mM [Na+]o (calculated by Clampex 10) and was corrected in (A–D). Image published in: Xue S et al. (2011) Xue et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page