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Figure 3. AtHKT1;1 mediates Na+ currents.A) Whole-cell current recorded with 50 mM NaCl in both the bath and pipette solutions in wild-type root stelar cells. B) Average current-voltage relationships recorded as in (A) from five protoplasts. Liquid junction potential (LJP) in (A and B) was +0.3 mV (calculated by Clampex 10) and was corrected in (A) and (B). C) Whole-cell current recorded with 5 mM NaCl in the pipette solution and 50 mM NaCl in the bath solution. D) Average current-voltage relationships recorded as in (C) from five protoplasts. LJP in (C and D) was +8 mV (calculated by Clampex 10) and was corrected in (C) and (D). ENa+: Na+ equilibrium potential. ECl−: Cl− equilibrium potential. The data in (B and D) are mean ± SE. The average currents of −19 and −35 pA activated at a voltage of −80 mV correspond to current densities of −11 and −21 mA m−2 with an average protoplast diameter of 23 µm. The bath solution in (A) to (D) contained 50 mM NaCl, 2 mM CaCl2, 5 mM MES, pH 5.7 adjusted with Tris. The pipette solution in (A) and (B) contained 50 mM NaCl, 1.3 mM CaCl2, 3 mM EGTA, 5 mM HEPES, pH 7.2 adjusted with Tris. The pipette solution in (C) and (D) contained 5 mM NaCl, 1.3 mM CaCl2, 3 mM EGTA, 5 mM HEPES, pH 7.2 adjusted with Tris. The osmolalities of bath and pipette solutions were adjusted to 290–300 mmol·Kg−1 with D-mannitol. Image published in: Xue S et al. (2011) Xue et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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