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Figure 5. Potassium currents in wild-type (WT-E2586) and athkt1;1-4 root stelar cell protoplasts are indistinguishable.A) Whole-cell currents activated by voltage ramps (5 mVms−1, +99 to −141 mV) with 5 mM KCl in the pipette solution and 50 mM KCl in the bath solution in wild-type (black trace) and athkt1;1-4 mutant (red trace) root stelar cells. B) Average current-voltage relationships recorded as in (A) from five athkt1;1-4 mutant protoplasts (open circles), five WT (E2586) protoplasts (filled circles). Average currents of −53 and −56 pA activated at a voltage of −141 mV correspond to current densities of −32 and −34 mA m−2 with an average protoplast diameter of 23 µm. The bath solution contained 50 mM KCl, 2 mM CaCl2, 5 mM MES, pH 5.7 adjusted with Tris. The pipette solution contained 5 mM KCl, 1.3 mM CaCl2, 3 mM EGTA, 5 mM HEPES, pH 7.2 adjusted with Tris. The osmolalities of bath and pipette solutions were adjusted to 290–300 mmol·Kg−1 with D-mannitol. Liquid junction potential (LJP) in (A and B) was +0.7 mV (calculated by Clampex 10) and was corrected in (A) and (B). Image published in: Xue S et al. (2011) Xue et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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