Xenbase Image ID: 126413

Figure 6. M-phase Cdk1 activity inhibits Rad51 loading on ssDNA-RPA. (A) Rad51 accumulates on resected chromatin, and Chk1 becomes activated in S- but not in M-phase extract. S- and M-phase extracts prepared from the same batch of eggs were either mock or CtIP depleted. Chromatin-binding time course in response to DSBs was performed as in Fig. 1 A. (B) Treatment of S-phase extract with Cdk1 inhibits Rad51 chromatin binding but not Chk1 activation. S-phase extract was supplemented with 100 nM recombinant Cdk1–cyclin B protein complex or buffer, and a chromatin-binding time course in response to DSBs was performed as in Fig. 1 A. (C) Inhibition of Cdk1 activity in M phase restores Rad51 accumulation in response to chromosomal DSBs. M-phase extract was supplemented with the specific Cdk1 inhibitor RO-3306 (200 µM) or DMSO, and a chromatin-binding time course in response to DSBs was performed as in Fig. 1 A. Time points above the blots are in minutes. Image published in: Peterson SE et al. (2011) © 2011 Peterson et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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