XB-IMG-126612
Xenbase Image ID: 126612
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Figure 3. Blockade of BDNF-induced growth cone turning by miR-134 mimic and antisense oligonucleotides. (A) Representative images of growth cones at the beginning and the end of 30 min exposure to a BDNF gradient. Dashed lines indicate the original direction of growth cone extension. Dotted lines indicate the position of the growth cone at the onset of the turning assay. Insets are fluorescent images of the same growth cones, showing the presence of the fluorescent tracer FITC-dextran co-injected with miR-134 mimic or antisense. (B) Trajectory tracings of all the growth cones subjected to 30 min turning assay in a BDNF gradient. Neurons injected with control oligonucleotides (Ctrl), miR-134 antisense inhibitors or mimics are shown here. The origin is the center of the growth cone at the onset of the BDNF gradient and the original direction of growth cone extension is vertical. Scale bars: 20 μm. Arrows indicate the direction of the BDNF gradient. (C) Average turning angles (top) and net extension (bottom) of different groups of all the growth cones examined. Numbers indicate the total number of growth cones examined for each group. Each group is labeled at the bottom: None: neurons not treated; Cyclo: bath application of cycloheximide; Ctrl: neurons injected with miR-134 control oligonucleotides; AS: injected with miR-134 antisense inhibitors; MM: injected with miR-134 mimics. Error bars represent the standard error of the mean. Asterisks depict the statistical significance (p < 0.01, Mann-Whitney test). Image published in: Han L et al. (2011) Copyright ©2011 Han et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |