Xenbase Image ID: 126663

Figure 1. ER remodeling and IP3 receptor clustering during meiosis.A. Functional clustering of elementary Ca2+ release events during oocyte maturation. Xenopus oocytes were injected with 10 µM caged IP3 and 40 µM Oregon-green. Oocyte maturation was induced with progesterone and both immature oocytes and fully mature eggs were imaged in linescan mode at 488 nm with the 405 nm laser at low intensity (0.2%) to continuously uncage cIP3. The same region in the cell was scanned continuously in linescan mode with the x-axis representing time and the y-axis space. The single isolated Ca2+ puffs observed in the oocyte coalesce into larger release events referred to as single release events (SRE). B. The ER remodels during oocyte maturation to form large patches in the egg. Oocytes were injected with GFP-IP3 receptor (IP3R) (50 ng/cell) and mCherry-KDEL (10 ng/cell). Images show the formation of ER patches in both animal and vegetal hemisphere to which IP3 receptors localize (Scale bar, 2 µm). C. Frequency of ER patches on the animal and vegetable poles (n = 166; 14 frogs). D. ER patch density and area (n = 38). E. Width of elementary Ca2+ release events in the egg as compared to the width of ER patches. Image published in: Sun L et al. (2011) Sun et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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