Xenbase Image ID: 126739

FIGURE 1. xCtc1, xStn1, and xTen1 form a trimeric complex. A. predicted structures of xCtc1, xStn1, and xTen1 gene products. Black boxes represent putative OB-fold-containing regions. Percentages of identical and similar amino acids shared by Xenopus proteins and human ones are summarized in the table. B, recombinant xCST (3×FLAG-xCtc1, His6-xStn1, and His6-xTen1) expressed in insect cells was affinity-purified using anti-FLAG beads and Ni beads. Proteins were visualized by silver staining. FT, flow-through fraction; B, bead-bound proteins after elution; E1 and E2, eluate with 100 mm imidazole; E3 and E4, eluate with 200 mm imidazole; and E5 and E6, eluate with 500 mm imidazole. C, E1 and E2 fractions in B were combined and subjected to gel filtration. Proteins in each fraction were separated by SDS-PAGE, and the gel was stained with Coomassie Brilliant Blue. Image published in: Nakaoka H et al. (2012) © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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