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FIGURE 2. xCST binds to ssDNA with moderate preference for G-rich sequences. A, preparation of recombinant xCST and xRPA used in EMSA. Proteins were visualized by SYPRO Ruby staining of SDS-polyacrylamide gel. B, sequences of 32-mer ssDNA probes used in EMSA. C, EMSA. The reaction mixtures included 20 nm 32P-labeled DNA probes and 10 nm xCST (lanes 6–10) or 10 nm xRPA (lanes 11–15). For lanes 1–5, control buffer was included instead of protein samples. Image published in: Nakaoka H et al. (2012) © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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