Xenbase Image ID: 126741

FIGURE 3. xStn1 associates with chromatin and localizes at telomeres. A, xStn1 localization in in vitro reconstituted Xenopus sperm chromatin was analyzed by indirect immunofluorescence in combination with telomere FISH. A representative set of microscopic images is shown. DNA stained with DAPI is shown in blue. White arrowheads indicate the co-localization of xStn1 foci (green) and telomeric FISH signals (red). Enlarged images of the numbered white boxes on the merged picture are shown on the right. B, Xenopus sperm nuclei were incubated in interphase LSS for the indicated times. The time when sperm nuclei were added to the egg extract was set to 0 min. Isolated chromatin was analyzed by immunoblotting using the indicated antibodies. The reaction mixtures contained control buffer (lanes 1–4), 60 μg/ml GST-geminin H (lanes 5–8), or 50 μg/ml aphidicolin (lanes 9–12), respectively. Image published in: Nakaoka H et al. (2012) © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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