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FIGURE 5. xCST(n) enhances M13 replication and reverses the neutralizing effect of anti-xStn1 antibody. A, M13 was incubated in HSS containing [α-32P]dCTP for 1.5 h in the presence of 0, 0.05, or 0.1 pmol/μl xCST(n). Replication efficiency, which was determined by measuring radioactivity on the gel, was normalized to the standard condition (without xCST(n) addition). The results of three independent experiments are shown in the bar graph. B, same experiment as A, except that anti-xStn1 KU004 was included in the reactions at the concentration of 0.04 μg/μl. The results of three independent experiments are shown in the bar graph. Replication efficiency was normalized to the standard condition (without xCST(n) and antibody addition).

Image published in: Nakaoka H et al. (2012)

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license

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