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FIGURE 6. Priming is disturbed in the absence of xStn1. Left, primed or nonprimed M13 was incubated for 1 h in xStn1-immunodepleted or mock-immunodepleted HSS containing [α-32P]dCTP. KU003 antibody was used to immunodeplete xStn1 (see “Experimental Procedures”). Right, radioactivity on the gel was quantified to calculate the amount of newly synthesized DNA in the reactions. Replication efficiency was defined as the ratio of the amount of synthesized DNA to that of input DNA and expressed as percentage. The graph shows the results of three independent experiments. Image published in: Nakaoka H et al. (2012) © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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