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Figure 2. Breeding strategy to generate double-transgenic hopper frogs. The F2 hopper frogs were outcrossed with wild-type animals and the progeny was scored for GFP and RFP expression. The GFP-positive/RFP-negative F3 progeny were either raised to adulthood for outcross or genomic DNA was harvested after stage 45 for molecular analyses. GFP: green fluorescent protein; RFP: red fluorescent protein. Image published in: Yergeau DA et al. (2011) Copyright ©2011 Yergeau et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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