Xenbase Image ID: 127090

Figure 7. Mouse MCI governs MCC differentiation in MTEC cultures(a–c) MTEC cultures were established, infected with lentivirus expressing MT-MCI, MT-MCIΔCC or GFP as a control, and switched to ALI culture conditions. At 4 days post-ALI, cultures were fixed, stained for infected cells (green) using an antibody to the myc-tag or GFP, to pericentrin (red) to identify MCCs and to E-cadherin (blue) to label cell boundaries. Shown are examples of cultures infected with a virus expressing GFP (a), MT-MCI (b) or MT-MCI-ΔCC (c). Arrows and asterisks denote infected cells that are pericentrin positive or negative, respectively. The insert in panel b shows a higher power image of pericentrin staining that marks clusters of nascent basal bodies. Scale bars = 50 microns (d) Percentage of infected cells that were ciliated versus nonciliated based on FoxJ1 or pericentrin staining under each condition, where n=total # of infected cells scored under each condition (±s.d.). Values marked with an asterisk are statistically significant (p<1×10 −10) from the values obtained for the GFP controls. Image published in: Stubbs JL et al. (2012) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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