Xenbase Image ID: 127220

Figure 1. Analysis of DPP6 splice variant expression in CG cells.A) DPP6 gene shows a conserved set of four alternative first exons producing the protein variants DPP6a, DPP6K, DPP6L and DPP6S. B) qRT-PCR using SYBR Green Fluorescence for DPP6 variants from three brain regions: cortex, cerebellum and hippocampus. DPP6a and DPP6K show enhanced expression in cerebellum. C) Normalization controls used to correct for primer amplification efficiency differences. Amplification targets from GAPDH and two DPLP variants were diluted and used to construct amplification curves. Common GAPDH signal was used to ensure consistent dilution of standards. D) Relative expression levels of DPP6 variants in cerebellum following normalization. Due to high levels of expression in CG cells and the abundance of these neurons, these signals essentially report the relative expression of DPP6 variants in CG cells. Image published in: Jerng HH and Pfaffinger PJ (2012) Jerng, Pfaffinger. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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