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Figure 5. Co-assembly of DPP6K and DPP6a in heteromultimeric channel complexes.(A) Outward currents expressed by oocytes co-injected by various combinations of cRNAs, as elicited by depolarization to +40 mV from holding potential of −100 mV. (B) Expected rise and decay of currents if DPP6a and DPP6K subunits do not co-assemble and produce segregated channel populations containing either one alone. (C) Slowing of the time constant of fast inactivation when DPP6a mRNA changes from 100% to 10% mixed with DPP6K mRNA. To get the average value for fast inactivation, the slow phase of inactivation and non-inactivating current were described by exponential fitting and subtracted from the total current. The remaining average fast inactivation time constant was measured by taking the peak current for the fast inactivating fraction divided by its area. The average time constant measured by this method was very similar to the time constant measured by the best single exponential fit to the fast inactivating component. The black and gray lines show the predicted maximal slowing of fast inactivation with four DPP6 and two DPP6 per channel, respectively, with only 1 DPP6a subunit per channel. (D) Recovery from inactivation at −100 mV after a 200 ms-long prepulse (symbols) as compared to predicted results assuming no co-assembly of DPP6a and DPP6K (dashes). Image published in: Jerng HH and Pfaffinger PJ (2012) Jerng, Pfaffinger. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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