Xenbase Image ID: 127297

Figure 2. In vivo imaging of IFT in Xenopus multiciliated cells. (a) Still frame from a video of GFP-IFT20 in a multiciliated cell (Video 1). The orange box indicates the region shown in the bottom image, depicting still frames from a time-lapse video showing processive bidirectional movement of IFT particles in a single control cilium (Video 2). Time is indicated in seconds. Pink arrowheads indicate anterograde particles; blue arrowheads indicate retrograde particles. The axoneme is outlined in yellow. Bars, 3 µm. (b) Histogram of anterograde rates of IFT in Xenopus multiciliated cells. (c) Histogram of retrograde rates. (c and d) Mean velocities ± SD are 0.84 ± 0.22 µm/s anterograde (n = 143 trains from 24 cells; six embryos) and 0.87 ± 0.22 µm/s retrograde (n = 125 trains from 25 cells; six embryos). Velocities ranged from 0.36 to 1.35 µm/s anterograde and 0.42 to 1.43 µm/s retrograde. Velocities were calculated as the mean of three independent instantaneous velocities for each reported particle. Image published in: Brooks ER and Wallingford JB (2012) © 2012 Brooks and Wallingford. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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