Xenbase Image ID: 127299

Figure 4. Fuz is required for the axonemal localization and dynamics of retrograde IFT. (a) Punctate localization of the IFT-A component GFP-IFT43 in control multiciliated cell axonemes (marked by memRFP). (b) Reduced GFP-IFT43 in the axonemes of Fuz KD multiciliated cells. (c) Still frame from a video of a control multiciliated cell expressing GFP-IFT43 (Video 9). The orange box indicates the region shown in the bottom image, depicting still frames from a video showing processive bidirectional transport in a single control cilium (Video 10). Time is indicated in seconds. Pink arrowheads indicate anterograde particles; blue arrowheads indicate retrograde particles. (d) A single frame from a time-lapse video of a Fuz KD multiciliated cell expressing GFP-IFT43. The image has been intentionally overexposed to bring out the faint background fluorescence of the axonemes. The orange box indicates the region shown in the bottom image, depicting still frames from a video showing loss of GFP-IFT43 dynamics in a single cilium from a Fuz KD multiciliated cell. The axoneme is outlined in yellow. Bars, 3 µm. Image published in: Brooks ER and Wallingford JB (2012) © 2012 Brooks and Wallingford. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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