Xenbase Image ID: 127300

Figure 5. Fuz is required for the localization of anterograde but not retrograde IFT proteins to peri-basal body pools in the apical cytoplasm. (a–d) Pools of GFP-IFT43 surrounding basal bodies marked by Centrin-RFP (a, right) in a control cell. Similar pools are observed for GFP-IFT20 (b). GFP-IFT43 pools show reduced enrichment at basal bodies in Fuz KD cells (c). However, GFP-IFT20 is still appropriately localized under the same conditions (d). Note that Fuz KD cells exhibit a second phenotype of basal body clustering (yellow arrowheads in b and d; also see Gray et al. [2009]). Bars, 3 µm. (e) Quantitative comparison of GFP-IFT43 localization in control (Ctl) and Fuz KD cells. Each data point represents the mean of the mean intensities of all GFP-IFT43 pools in a cell normalized to the mean of the mean intensities of all Centrin foci. Fuz KD leads to a significant reduction in GFP-IFT43 localization to apical pools (Fuz KD [mean ± SD = 0.47 ± 0.20; n = 21 cells; six embryos] vs. control [mean ± SD = 0.67 ± 0.12; n = 21 cells; six embryos; P = 0.0003]). (f) A similar analysis of GFP-IFT20 shows no significant difference in localization between control and Fuz KD cells (Fuz KD [mean ± SD = 0.65 ± 0.20; n = 24 cells; six embryos] vs. control [mean = 0.66 ± 0.15; n = 22 cells; six embryos; P = 0.5308]). (g) A schematic model of IFT in control and Fuz KD axonemes. Image published in: Brooks ER and Wallingford JB (2012) © 2012 Brooks and Wallingford. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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