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Figure 5. Ct domains act cooperatively to affect K2P2.1 (TREK-1) function. (A) Immunoblot analysis of lysates from oocytes expressing HA-tagged WT, HA-WT or tandem HA-WT-WT K2P2.1 (TREK-1) channels. Lysates were pre-incubated with or without EndoH for 1 h at 4oC or at room temperature (RT). Before electrophoresis, all samples were treated with 2% SDS and 2% β-mercaptoethanol for 15 min at 50oC to dissociate K2P2.1 (TREK-1) subunits. Asterisks denote deglycolyslated forms of WT and tandem channels. (B–D) Normalized responses of the indicated K2P2.1 (TREK-1) channels to pHo changes 2 mM [K+]o. Currents were elicited by a voltage ramp from −150 to +50 mV, from a holding potential of −80 mV. Data (mean±s.e., n⩾6, N⩾2) was taken at 0 mV, normalized to activity at pH 9.0 and fitted to the Hill equation. Image published in: Bagriantsev SN et al. (2012) Copyright © 2012, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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