Xenbase Image ID: 127403

Figure 6. Both C-terminal domains are required for the K2P2.1 (TREK-1) temperature response. (A–C) Exemplar two-electrode voltage clamp recordings of K2P2.1 (TREK-1) (A), K2P2.1-3G (B), and K2P2.1-3A (C) responses to temperature in 2 mM [K+]o pH 7.4. Currents were elicited by a ramp from −150 to +50 mV, from a −80 mV holding potential. (D–F) Quantification of the temperature responses. Data (mean±s.e., n⩾6, N⩾2) was taken at 0 mV and normalized to channel activity at 14°C. (G) Cartoon model of how Ct couples to the C-type gate of K2P2.1 (TREK-1). M4, transmembrane segment 4. Channel elements come from a single subunit. Transmembrane segments M1–M3 and pore helix 2 are not depicted. Dashed regions indicate connections to parts of the subunit that are not shown. Green circles represent potassium ions in the selectivity filter. Image published in: Bagriantsev SN et al. (2012) Copyright © 2012, European Molecular Biology Organization. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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