Xenbase Image ID: 127444

Figure 2. PTX-sensitive activation of Kir3.1* by opsin and all-trans-retinal, or light-activated rhodopsin. (A) Xenopus oocytes were injected with opsin and Kir3.1* mRNAs. Current amplitude was recorded at −50 mV. Black bars represent the average current measured prior to all-trans-retinal application or in the dark (after 11-cis retinal incubation) in the case of light activation. White bars represent the average current induced by 5 µM all-trans-retinal or light stimulation, respectively. Numbers above bars denote the number of oocytes tested. (B) Percent change in current induced by application of either 5 µM all-trans-retinal or light (after 11-cis retinal incubation) in control (black bars) and in the presence of co-expressed catalytic subunit S1 of pertussis toxin (PTX-S1) (white bars). Changes in current were computed for each oocyte and then averaged (The resulting average changes are different from the changes in average current represented in panel A). (C) Concentration-dependent response to all-trans-retinal. Average data computed as in panel B. Line corresponds to Hill equation fit with h = 4 and EC50 = 2.5 µM. Each point represents the average of 7 to 40 measurements. Image published in: Caro LN et al. (2012) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page