Xenbase Image ID: 127589

Figure 7. Ectodermin/Tif1γ is epistatic to Par1b for regulation of TGFβ signaling. (a) Immunoblots for p21Waf1, JunB and PAI1, whose induction by TGFβ is inhibited by Par1b depletion but rescued by dual depletion of Par1b and Ecto. LaminB serves as loading control. (b and c) Panels show in situ hybridization of Xenopus embryos injected at the four-cell stage in the marginal zone with control MO (140 ng), Par1b MO (x+y, 80 ng total) alone or in combination with Ecto MO 29 (60 ng). Embryos were stained for Xbra and VegT. Note that Ecto depletion rescues gene expression in Par1b-depleted embryos (n=22, 77% and n=21, 67%) Image published in: Mamidi A et al. (2012) Copyright © 2012 Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 mesoderm marginal zone vegt.L antipodean, brat, tVegT, vegt-a, vegt-b, xombi X. laevis Throughout NF stage 10.5 marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + mark2 MO + NF10.5 (in situ hybridization) fig.7.b Expression Phenotype decreased amount tbxt.S expression in marginal zone Xla Wt + mark2 MO + NF10.5 (in situ hybridization) fig.7.c Expression Phenotype decreased amount vegt.L expression in marginal zone Larger Image Printer Friendly View

Return to previous page