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Xenbase Image ID: 127723
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Figure 2. Early redox and SFK signaling regulates late epimorphic regeneration. (A) Diagram of regeneration assays using zebrafish larval tail fins. (B) Quantification of regenerated tail fin length at 3 d after wounding (DMSO: 19 larvae; DPI: 20 larvae; PP2: 19 larvae). (C) Representative pictures used for quantification in B. Dotted lines indicate tail transection. (D) Quantification of blastemal proliferation at 36 h after wounding (DMSO: 19 larvae; DPI: 19 larvae; PP2: 21 larvae). Mitotic cells were detected using an antibody for phosphorylated histone H3 (H3P). (E) Representative pictures used for quantification in D. (F) Quantification of neutrophil recruitment to wounded fins at 3 d after wounding (DMSO: 30 larvae; DPI: 25 larvae; PP2: 25 larvae). (G) Representative pictures of Sudan black staining used for quantification in F. (H) Quantification of regenerated tail fin length at 3 d after wounding in control larvae and pu.1 MO-injected larvae (control/DMSO: 20 larvae; control/DPI: 13 larvae; control/PP2: 19 larvae; pu.1 MO/DMSO: 20 larvae; pu.1 MO/DPI: 20 larvae; pu.1 MO/PP2: 20 larvae). (I) Fluorescent pictures of 2.5-dpf Tg(mpx:Dendra2), which expresses Dendra2 specifically in neutrophils. (J) Sudan black staining of control larvae and pu.1 MO-injected larvae at 2.5 dpf. *, P < 0.05; one-way ANOVA with Dunnett’s posttest. Horizontal lines indicate means. Bars: (C, E, and G) 50 µm; (I and J) 100 µm. Image published in: Yoo SK et al. (2012) © 2012 Yoo et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |