Xenbase Image ID: 127739

Figure 4. Cell surface expression of CNGA1-GFP channels.A. Representative Western blot of total CNGA1-GFP protein (TP) and biotinylated CNGA1-GFP cell surface protein (SP). WT, R272C, R272C+MTSEA denote cysteine-less CNGA1-GFP, mutant channels that were not treated with MTSEA and mutant channels that were modified by MTSEA, respectively. An expected band of ∼106 kDa for the deglycosylated wild-type GFP tagged channel was detected by chemiluminiscence using a GFP antibody. No signal was detected in non injected oocytes. B. Densitometry analysis of the bands normalized to TP (n = 3 different oocytes batches). Image published in: Lopez-Rodriguez A and Holmgren M (2012) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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