Xenbase Image ID: 127740

Figure 5. Y176C CNGA1-GFP mutant channels.A. Confocal image of an oocyte injected with cRNA encoding for Y176C CNGA1-GFP channels. We never detected any signs of proper trafficking in >100 oocytes by standard fluorescence microscopy, suggesting that these channels do not reach the cell surface. B. MTSHB treatment recovers proper trafficking of Y176C mutant channels to the cell surface. Confocal image shows GFP fluorescent signal at the cell surface of an oocyte expressing Y176C mutant channels. MTSHB was injected ∼ 12 h before acquiring the image (n >25 oocytes). C. Ionic currents in the presence of a saturating cGMP concentration (2 mM), in response to the same voltage protocol as described in Fig. 3A. D. Dose response for cGMP at +60 mV. Solid line represents a normalized Hill equation fit to the data. The best fit parameter values for K½ and n were 16±1 µM and 1.30±0.05, respectively. From a total of 8 oocytes, the average values for K½ and n were 16±3 µM and 1.6±0.1, respectively. E. Agonist efficacies of rescued Y176C mutant channels. Nucleotide-activated current records shown in the presence of saturating concentrations of each agonist (same as Fig. 3C) were acquired from the same excised patch. Rescued Y176C channels showed a similar efficacy pattern as cysteine-less CNGA1-GFP channels. Similar observations were observed in 4 patches. Image published in: Lopez-Rodriguez A and Holmgren M (2012) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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