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Figure 5. Determination of the apparent molecular weight of CAF-1/H3-H4 complex using SEC-MALS. (A) The elution profiles for CAF-1 (gray) and H3-H4 bound CAF-1 (black). H3-H4 binding by CAF-1 results in earlier elution volumes (10 ml) than CAF-1 alone (12 ml), suggesting a larger molecular weight for the complex. Light scattering confirms a consistently larger molecular weight across the earlier eluting peak (Panel A, right axis and Table 2). (B) The same size exclusion peak profile as (A), but with the measured root mean square (RMS) radius for each peak highlighted on the right axis. The CAF-1/H3-H4 complex has only a slightly larger radius in solution than CAF-1 alone (Table 2). Image published in: Winkler DD et al. (2012) © The Author(s) 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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