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Figure 6. Two H3-H4 molecules are co-purified with CAF-1 in yeast cells. (A–B) CAF-1 binds both H3 and H3-HA. The sequential IP procedure is outlined in panel A. Cac2-TAP is first purified from a yeast strain with either HHT1 or HHT2 tagged with the HA epitope using IgG sepharose.The eluted proteins are either immunoprecipitated with antibodies against the HA epitope (Panel B, lanes 1–3) or precipitated with TCA (lanes 4–6). After precipitation, H3 and H3-HA were detected by western blot using antibodies against H3K56Ac and both the HA-tagged and untagged H3 were found in complex with CAF-1. Image published in: Winkler DD et al. (2012) © The Author(s) 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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