Xenbase Image ID: 127838

Fig 1. Using EST assemblies to accurately define transcript sequences. (a) EST contigs for a pair of Xenopus laevis homeolog genes (H+transporting F1 ATP synthase, epsilon subunit, atp5e), illustrating sensitivity of clustering and accuracy of assembly. The EST assembly for each homeolog is shown, where the consensus sequence is constructed from the aligned EST sequences. Protein alignments are used to determine the frame and position of the ORF. (b) BLASTn alignment of the open reading frames (plus 12 bases upstream) of these two genes illustrates how important knowing both these sequence is to the process of morpholino design. The Gurdon IDs for the assemblies for the A and B homeologs are Xl2.1-LANE.XL433b16.5 and Xl2.1-Ls19H.BX846122.5, respectively. Image published in: Gilchrist MJ (2012) Copyright © 2012 Wiley Periodicals, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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