Xenbase Image ID: 127927

Fig. 1. Dgp71WD120 is a null mutant.(A) Schematic view of the Dgp71WD gene. Coding sequences are indicated in green and UTRs in blue; the inverted triangle represents the insertion site of the Dgp71WDGE30807 P-element. The dotted line represents the region deleted in the Dgp71WD120 mutant. Coloured arrows refer to PCR primer pairs used for the PCRs shown in (B) - red  =  reaction (i), purple  =  reaction (ii). (B) Agarose gel of the PCR products obtained using the indicated primer pairs (the final reaction is a positive control) using either WT or Dgp71WD120 genomic DNA. (C) Western blots of WT and Dgp71WD120 3rd instar larval brain extracts at two different exposure levels. In WT extracts a double band can be seen below the 84 kDa marker. The higher band is nonspecific-background whereas the lower band represents Dgp71WD. The lower band is absent in the mutant, indicating that Dgp71WD120 is likely a protein null. (D) WT and Dgp71WD120 3rd instar larval brain cells stained with anti-Cnn (green) and anti-Dgp71WD (red) antibodies; DNA (blue). Scale bar represents 2.5 µm. Image published in: Reschen RF et al. (2012) © 2012. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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