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Figure 3. The T930E mutation decreases Ins(1,4,5)P3 binding affinity. Microsomal preparations from brain as a positive control and from the different DT40 cell lines as indicated were subjected to the Ins(1,4,5)P3 binding assay to measure the binding affinity of Ins(1,4,5)P3 receptor mutants. Briefly lysates were allowed to bind radioactively (3H) labeled Ins(1,4,5)P3 and the label competed with cold Ins(1,4,5)P3. The competitive assay provides a relative comparative measure Ins(1,4,5)P3R affinity in the different mutants as compared with the wild type receptor (WT). The negative control was the 3KO cell line which does not express any of the three Ins(1,4,5)P3 receptor isoforms and as such provides non-specific background binding. Image published in: Haun S et al. (2012) Copyright © 2012 Landes Bioscience. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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