XB-IMG-128022
Xenbase Image ID: 128022
|
Figure 7. Transactivation of the transgenic TRE:HA-Crb2aIntraWT.(A) Larvae from an outcross of Tg(Xla.rho:rtTA, TRE:GFP) to Tg(TRE:HA-Crb2aIntraWT) were untreated or doxycycline (Dox)-treated for 72 hours and genotyped for the transgenes before immunofluorescence analysis. (B–J) Confocal z-projections of retinal sections from 6 dpf Tg(Xla.rho:rtTA, TRE:GFP; TRE:HA-Crb2aIntraWT) larvae labeled with anti-HA (red) and anti-Rhodopsin (blue) antibodies. (B–D) Anti-HA-Crb2aIntraWT immunofluorescence (B, red) and GFP fluorescence (C, green) are undetectable in the untreated rod photoreceptors with anti-Rhodopsin immunofluorescence (D, blue). (E–J) Anti-HA-Crb2aIntraWT immunofluorescence (E, H, red) and GFP fluorescence (F, I, green) are visible in the photoreceptor layer and co-localize with the anti-Rhodopsin immunofluorescence in the rod photoreceptors after 72 h of Dox treatment. Scale bar (D, G), 50 µm and (J), 20 µm. Image published in: Campbell LJ et al. (2012) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |