Xenbase Image ID: 128490

Fig. 2. Effective b2m silencing in vivo in transgenic F1 OB and LG Xenopus clones.RT-PCR was performed on RNA from the skin of young adults (2 months of age) OB F1 transgenic (GFP+) carrying either the anti-b2m shRNA (OB-Tg, 9 individuals), the scram shRNA transgene (Sc, 6 individuals) and RNA from skin of LG-6 anti-b2m shRNA transgenic (LG6-Tg, 9 individuals). RT-PCR analysis was performed using primers specific for Xenopus GAPDH and b2m. −RT controls for all samples were negative. The percent of b2m KD was determined by densitometry after normalization to GAPDH by comparing b2m expression of each transgenic to the average expression in the respective WT controls. Average ± s.d. of b2m is also indicated by filled symbols for each group of transgenic. Statistical significance between controls and transgenic animals was determined with a t-test. Image published in: Nedelkovska H et al. (2013) © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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