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Fig. 4. LG-6 clones with silenced b2m expression have reduced frequency of circulating CD5/CD8 T cells in the spleen and the blood.Spleen and blood were harvested from two transgenic LG-6 froglets with silenced b2m expression as well as an age matched LG-6 naïve animal. Cells were stained for CD5 and CD8 and were analyzed by flow cytometry. 10,000 events were collected and were gated on live leukocytes. Analysis was performed using the FlowJo software. Shown are dot plots of CD5 and CD8 expression with % of each population labeled. Image published in: Nedelkovska H et al. (2013) © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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