XB-IMG-128511
Xenbase Image ID: 128511
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Figure 6. Both N- and C-terminal regions of CNGA3 subunits are important for modulation of heteromeric CNGA3+CNGB3 channels by PIP3 or PIP2. (A) Representative cGMP concentration–response plot showing the effect of 10 µM PIP2 on A3+B3 heteromeric channels having N- (N7R-A) and C-terminal (RR-QQ) charge neutralizations within CNGA3. (B) Effects of PIP3 (1 µM) and PIP2 (10 µM) on heteromeric A3+B3 channels having specified mutations in A3 subunits. *, P < 0.05; **, P < 0.001 compared with wild-type A3+B3 for both PIP3 and PIP2. In the pairwise comparisons, statistically significant differences (P < 0.05) were also detected between A3N7R-A+B3 or A3 RR-QQ+B3 versus A3 N7R-A, RR-QQ+B3 channels. (C) Representative cGMP concentration–response relationship for activation of A2ΔN+B3 channels before (black) and after 1 µM PIP3 (red). (D) Percentage change in K1/2 cGMP after PIP3 (1 µM) for various CNG channel subunit compositions. A2ΔN (Brady et al., 2006), A2ΔN+B1, A2ΔN+B3 channels showed no sensitivity to PIP3, whereas PIP3 produced a statistically significant increase in K1/2 cGMP for A1+B3 (n = 4), A2+B3 (n = 3), and A3+B1 (n = 4) channels compared with A2ΔN+B3 or A2ΔN+B1 channels (*, P < 0.05). Error bars indicate mean ± SEM. Image published in: Dai G et al. (2013) © 2013 Dai et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |